Journal:

Article Title: Hybrids of Dendritic Cells and Tumor Cells Generated by Electrofusion Simultaneously Present Immunodominant Epitopes from Multiple Human Tumor-Associated Antigens in the Context of MHC Class I and Class II Molecules 1

doi:

Figure Lengend Snippet: DC phenotype before electrofusion. DCs from donor 4 were stained with FITC- (x-axis; FL1-H) and PE- (y-axis; FL2-H) conjugated mAbs as indicated and analyzed by FACS.

Article Snippet: After fusion, DC-888mel hybrids were detected by staining with PE-conjugated mAbs against DC cell surface markers including CD11c, CD40, CD80, CD86, CD83, and HLA-DR (BD PharMingen, San Diego, CA).

Techniques: Electrofusion, Staining

Journal:

Article Title: Hybrids of Dendritic Cells and Tumor Cells Generated by Electrofusion Simultaneously Present Immunodominant Epitopes from Multiple Human Tumor-Associated Antigens in the Context of MHC Class I and Class II Molecules 1

doi:

Figure Lengend Snippet: FACS analyses of DC-TC hybrid cells generated by electrofusion. 888 melanoma cells were labeled with CFSE (x-axis; FL1-H) and were fused with DCs from donor 4. After overnight culture, both adherent and nonadherent cell populations were stained with PE- (y-axis; FL2-H) conjugated mAbs as indicated.

Article Snippet: After fusion, DC-888mel hybrids were detected by staining with PE-conjugated mAbs against DC cell surface markers including CD11c, CD40, CD80, CD86, CD83, and HLA-DR (BD PharMingen, San Diego, CA).

Techniques: Generated, Electrofusion, Labeling, Staining